Molecular biology topics for USMLE Step 1
Molecular biology topics for USMLE Step 1
Biochemistry and molecular biology
- gene expression: DNA structure, replication, and exchange
- DNA structure: single- and double-stranded DNA, stabilizing forces, supercoiling
- analysis of DNA: sequencing, restriction analysis, PCR amplification, hybridization
- DNA replication, mutation, repair, degradation, and inactivation
- gene structure and organization; chromosomes; centromere, telomere
- recombination, insertion sequences, transposons
- mechanisms of genetic exchange, including transformation, transduction, conjugation, crossover, recombination, linkage
- plasmids and bacteriophages
- gene expression: transcription, including defects
- transcription of DNA into RNA, enzymatic reactions, RNA, RNA degradation
- regulation: cis-regulatory elements, transcription factors, enhancers, promoters, silencers, repressants, splicing
- gene expression: translation, including defects
- the genetic code
- structure and function of tRNA
- structure and function of ribosomes
- protein synthesis
- regulation of translation
- post-translational modifications, including phosphorylation, addition of CHO units
- protein degradation
- structure and function of proteins
- principles of protein structure and folding
- enzymes: kinetics, reaction mechanisms
- structural and regulatory proteins: ligand binding, self-assembly
- regulatory properties
source: http://usmle.org/Examinations/step1/content/principles.html
Categorised as: Molecular Biology 1